Bioactive Natural Products from Polyalthia Longifolia and its Fungal Endophyte Alternaria sp.

Walid Amir Sabry; Mahmoud Fahmi Elsebai; Mohamed Amir Sabry; Saleh Hassan Elsharkawy; Ashraf Taha Khalil

Authors

Walid Amir Sabry Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt
Mahmoud Fahmi Elsebai Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University, Mansoura-35516, Egypt. Department of Natural Products and Alternative Medicine, Faculty of Pharmacy, University of Tabuk, Tabuk, Kingdom of Saudi Arabia.
Mohamed Amir Sabry Department of Medicinal Chemistry, Faculty of Pharmacy, Mansoura University, Mansoura-35516, Egypt.
Saleh Hassan Elsharkawy Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University, Mansoura-35516, Egypt.
Ashraf Taha Khalil Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University, Mansoura-35516, Egypt.

Keywords:

Polyalthia longifolia, Fungal endophyte, Clerodane diterpenes, Alternaria, Cytotoxicity, Carbonic anhydrase

Abstract

Medicinal plants and fungi are rich sources of natural products. The plant Polyalthia longifolia was investigated for its phytochemical components and its own endophytes. Phytochemical investigation of the aerial parts of P. longifolia has led to the isolation and identification of β-sitosterol-3-O-β-D-glucoside (1), 14,15-bisnor-3,11-E-kolavadien-13-one (2), 3-β,5-β,16-α-trihydroxyhalima-13(14)-en-15,16-olide (3), isoquercetin (4), rutin (5), and trans-cinnamic acid (6). Furthermore, the endophyte Alternaria sp. (MT995125) was isolated from the leaves and the compounds pyrenolide-D (7) and modiolide-A (8) were identified in its solid culture. The isolated compounds were tested against human epithelioid cervical carcinoma (HeLa), human colorectal carcinoma (HCT-116) and human amnion (WISH) normal cell line. The compounds showed moderate to strong cytotoxic activity with minimal toxicity towards normal cell line WISH compared to the positive control doxorubicin. The significant cytotoxic activities of some compounds were augmented by in silico strong inhibitory activity to carbonic anhydrase (IX isoform) (CA IX) through performing docking experiment.