Chemical Screening, Determination of Polyphenols and Total Flavonoids, Antioxidant activity of extracts from the leaves of Dischistocalyx hirsutus (Acanthaceae)

https://doi.org/10.54037/WJPS.2021.91003

Authors

  • G. S. A. Amboyi Laboratory of Applied Chemistry Research (LARCA), Department of Exact Sciences, Ecole Normale Supérieure (ENS), University Marien Ngouabi, Brazzaville-CONGO.
  • T. Andzi Barhé Laboratory of Applied Chemistry Research (LARCA), Department of Exact Sciences, Ecole Normale Supérieure (ENS), University Marien Ngouabi, Brazzaville-CONGO.
  • A. B. Boukongou Laboratory of Applied Chemistry Research (LARCA), Department of Exact Sciences, Ecole Normale Supérieure (ENS), University Marien Ngouabi, Brazzaville-CONGO.

Keywords:

Dischistocalyx hirsutus, Chemical Screening, polyphenolic content, flavonoid content, Antioxidant activity

Abstract

This article presents the phytochemical composition, the polyphenols and total flavonoids content, and the antioxidant activity of the extracts of the leaves from Dischistocalyx hirsutus. The phytochemical test was carried out thanks to the colorimetry and precipitation reactions. The total phenol content was determined using the Folin-Ciocalteu reagent and that of the flavonoids by reaction with AlCl3. There were three extracts:  aqueous, hydro-ethanolic and ethanolic extracts. Antioxidant activity was assessed by DPPH methods. The results showed that the extracts of Dischistocalyx hirsutus contain large chemical groups, such as flavonoids, tannins, anthocyanins and saponosides. The total phenol contents are 76.5 ± 0.2; 66.5 ± 0.1 and 55.3 ± 0.1 mg EGA/gMS, respectively for the hydro-ethanolic, ethanolic and aqueous extracts. the contents of total flavonoids are 5.5 ± 0.1; 4.5 ± 0.1 and 3.3 ± 0.1 mg EC/gMS. Color intensities (IC50) of 27.5, 17 and 17.5 are obtained, respectively for the aqueous, hydro-ethanolic and ethanolic extracts. These results highlight the interest of this plant and justify its use in traditional medicine for the treatment of certain pathologies.

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Published

2021-10-01

How to Cite

G. S. A. Amboyi, T. Andzi Barhé, & A. B. Boukongou. (2021). Chemical Screening, Determination of Polyphenols and Total Flavonoids, Antioxidant activity of extracts from the leaves of Dischistocalyx hirsutus (Acanthaceae): https://doi.org/10.54037/WJPS.2021.91003. World Journal of Pharmaceutical Sciences, 9(10), 10–16. Retrieved from http://wjpsonline.com/index.php/wjps/article/view/antioxidant-activity-leaves-dischistocalyx-hirsutus

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Research Article

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