Chemical Screening, Determination of Polyphenols and Total Flavonoids, Antioxidant activity of extracts from the leaves of Dischistocalyx hirsutus (Acanthaceae)
Keywords:Dischistocalyx hirsutus, Chemical Screening, polyphenolic content, flavonoid content, Antioxidant activity
This article presents the phytochemical composition, the polyphenols and total flavonoids content, and the antioxidant activity of the extracts of the leaves from Dischistocalyx hirsutus. The phytochemical test was carried out thanks to the colorimetry and precipitation reactions. The total phenol content was determined using the Folin-Ciocalteu reagent and that of the flavonoids by reaction with AlCl3. There were three extracts: aqueous, hydro-ethanolic and ethanolic extracts. Antioxidant activity was assessed by DPPH methods. The results showed that the extracts of Dischistocalyx hirsutus contain large chemical groups, such as flavonoids, tannins, anthocyanins and saponosides. The total phenol contents are 76.5 ± 0.2; 66.5 ± 0.1 and 55.3 ± 0.1 mg EGA/gMS, respectively for the hydro-ethanolic, ethanolic and aqueous extracts. the contents of total flavonoids are 5.5 ± 0.1; 4.5 ± 0.1 and 3.3 ± 0.1 mg EC/gMS. Color intensities (IC50) of 27.5, 17 and 17.5 are obtained, respectively for the aqueous, hydro-ethanolic and ethanolic extracts. These results highlight the interest of this plant and justify its use in traditional medicine for the treatment of certain pathologies.
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