Method development and validation for simultaneous estimation of netupitant and palonosetron in bulk and pharmaceutical dosage form by using RP-HPLC

https://doi.org/10.54037/WJPS.2021.91212

Authors

  • Sridhar Siddiraju Department of Pharmaceutical Chemistry, Malla Reddy College of Pharmacy, Osmania University, Secunderabad - 500010, Telanagana, India
  • Akshay Department of Pharmaceutical Analysis, Malla Reddy College of Pharmacy, Osmania University, Secunderabad - 500010, Telanagana, India
  • M. Sudhakar Department of Pharmaceutics, Malla Reddy College of Pharmacy, Osmania University, Secunderabad - 500010, Telanagana, India

Keywords:

Netupitant, Palonosetron, Method development, RP-HPLC

Abstract

A simple, Accurate, precise method was developed for the simultaneous estimation of the Netupitant and Palanosetron in Tablet dosage form. Chromatogram was run through Std Discovery C18 250 x 4.6 mm, 5. Mobile phase containing Buffer 0.1% OPA (2.2ph): Acetonitrile taken in the ratio 55:45 was pumped through column at a flow rate of 1 ml/min. Buffer used in this method was 0.1% OPA. Temperature was maintained at 30°C. Optimized wavelength selected was 230 nm. Retention time of Netupitant and Palanosetron were found to be 2.325min and 3.026min. %RSD of the Netupitant and Palanosetron were and found to be 0.9 and 0.6 respectively. %Recovery was obtained as 100.28% and 99.76% for Netupitant and Palanosetron respectively. LOD, LOQ values obtained from regression equations of Netupitant and Palanosetron were 1.01, 3.05 and 0.001, 0.003 respectively. Regression equation of Netupitant is y = 13900x + 26511, and y = 563617x + 1740.8 of Palanosetron. Retention times were decreased and that run time was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.

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Published

2021-12-01

How to Cite

Sridhar Siddiraju, Akshay, & M. Sudhakar. (2021). Method development and validation for simultaneous estimation of netupitant and palonosetron in bulk and pharmaceutical dosage form by using RP-HPLC: https://doi.org/10.54037/WJPS.2021.91212. World Journal of Pharmaceutical Sciences, 9(12), 218–225. Retrieved from https://wjpsonline.com/index.php/wjps/article/view/estimation-netupitant-palonosetron-hplc

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Section

Research Article

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