Stability Indicating Method Development and Validation for Simultaneous Estimation of the Netupitant and Palonosetron in Bulk and Pharmaceutical Dosage Form by RP-HPLC

https://doi.org/10.54037/WJPS.2022.100112

Authors

  • Harshini Gandla M. Pharmacy Scholar, Department of Pharmaceutical Analysis, Institute of Science and Technology, JNTUH, Hyderabad
  • M. Ajitha Professor & Head, Deputy Director of Academic Audit Cell - JNTUH & OIE, Center for Pharmaceutical Sciences, JNTUH, Hyderabad

Keywords:

Netupitant, Palonosetron, RP-HPLC

Abstract

A simple, Accurate, precise method was developed for the simultaneous estimation of the Netupitant and Palonosetron in Pharmaceutical dosage form. Chromatogram was run through Phenomenex C18 150 mm (4.6 x 150mm, 5µm) Mobile phase containing Buffer 60% 0.01N KH2PO4: 40% Acetonitrile was pumped through column at a flow rate of 1 ml/min. Temperature was maintained at 30°C. Optimized wavelength selected was 230.0 nm. Retention time of Palonosetron and Netupitant were found to be 2.266 min and 2.945 min. %RSD of the Netupitant and Palonosetron were and found to be 0.8 and 0.8 respectively. %Recovery was obtained as 101.08% and 100.35%for Netupitant and Palonosetron respectively. LOD, LOQ values obtained from regression equations of Netupitant and Palonosetron were 1.27, 3.86 and 0.002, 0.006. respectively. Regression equation of Netupitant is y =18431x + 50471.and y = 13091x + 11.98. of Palonosetron. Retention times were decreased and that run time was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.

Downloads

Published

2022-01-02

How to Cite

Harshini Gandla, & M. Ajitha. (2022). Stability Indicating Method Development and Validation for Simultaneous Estimation of the Netupitant and Palonosetron in Bulk and Pharmaceutical Dosage Form by RP-HPLC: https://doi.org/10.54037/WJPS.2022.100112. World Journal of Pharmaceutical Sciences, 10(01), 113–120. Retrieved from https://wjpsonline.com/index.php/wjps/article/view/stability-indicating-estimation-netupitant-palonosetron-hplc

Issue

Section

Research Article

Most read articles by the same author(s)