Stability Indicating RP-HPLC Method Development and Validation for simultaneous estimation of Azelnidipine and Telmisartan in Bulk and Pharmaceutical Dosage Form
Keywords:Azelnidipine, Telmisartan, RP-HPLC
A simple, Accurate, Precise method was developed for the simultaneous estimation of Azelnidipine and Telmisartan in bulk and pharmaceutical dosage form by RP- HPLC technique. Chromatogram was run through Std Denali C18 150mm x 4.6 mm, 5µ. Mobile phase 0.1% OPA: Acetonitrile in the ratio of 60:40 and flow rate were maintained at 1.0 ml/min. Buffer used in this method was 0.1%OPA. Column temperature was set to 30oC.Optimised wavelength selected was 242.0nm. Retention time of Azelnidipine and Telmisartan were found to be 2.116 min & 3.188mins. %RSD of the Azelnidipine and Telmisartan System were found to be 1.6% and 1.0% respectively. %Recovery was obtained as 100.15% and 100.20% for Azelnidipine and Telmisartan respectively. LOD and LOQ values obtained from regression equations of Azelnidipine and Telmisartan were 0.04,0.13 and 0.38,1.14 respectively. Regression equation of Azelnidipine and Telmisartan is Y= 36260x+2218 and Y=30420x+8163.Retention times were decreased and that run time was decreased, so the method developed was simple and economical that can be adopted in regular quality control tests in industries.
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