Stability indicating RP-HPLC method development and validation for the simultaneous estimation of vilanterol and umeclidinium bromide in bulk and pharmaceutical dosage forms

https://doi.org/10.54037/WJPS.2022.100114

Authors

  • Diviti Sai Sindhu M. Pharmacy Scholar, Department of Pharmaceutical Analysis, Institute of Science and Technology, JNTUH, Hyderabad
  • S. Shoba Rani Associate Professor, NSS Co-Ordinator & PIU at Centre for Pharmaceutical Sciences, Institute of Science and Technology, JNTUH, Kukatpally, Hyderabad, Telangana

Keywords:

Vilanterol, Umeclidinium bromide, RP-HPLC

Abstract

A simple, Accurate, precise method was developed for the simultaneous estimation of the Vilanterol and Umeclidinium bromide in dosage form. Chromatogram was run through BDS C18 (150 x 4.6 mm, 5.0m). Mobile phase containing Buffer 0.1% Formic acid: Acetonitrile taken in the ratio 65:35 was pumped through column at a flow rate of 1.0ml/min. Temperature was maintained at 30°C. Optimized wavelength selected was 265 nm. Retention time of Umeclidinium and Vilanterol were found to be 2.363 min and 3.101 min. %RSD of the Vilanterol and Umeclidinium bromide were and found to be 0.4and0.7respectively. %Recovery was obtained as 99.49%and 101.14% for Vilanterol and Umeclidinium bromide respectively. LOD, LOQ values obtained from regression equations of Vilanterol and Umeclidinium bromide were 0.19, 0.56 and 0.59, 1.8 respectively. Regression equation of Vilanterol is y = 69945x + 7045 and y = 89939x + 60718 of Umeclidinium bromide. Retention times were decreased and that runtime was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.

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Published

2022-01-02

How to Cite

Diviti Sai Sindhu, & S. Shoba Rani. (2022). Stability indicating RP-HPLC method development and validation for the simultaneous estimation of vilanterol and umeclidinium bromide in bulk and pharmaceutical dosage forms: https://doi.org/10.54037/WJPS.2022.100114. World Journal of Pharmaceutical Sciences, 10(01), 128–135. Retrieved from http://wjpsonline.com/index.php/wjps/article/view/stability-indicating-hplc-estimation-vilanterol-umeclidinium

Issue

Section

Research Article