Antioxidant potential of arjunolic acid in an in-vitro system of precision cut goat liver slices subjected to oxidative stress

Abstract

Oxidative stress upshots from imbalance between antioxidant defense system and reactive oxygen species. It often leads to various pathological diseases like cancer, ischemia, inflammation, atherosclerosis and other neurodegenerative disorders. Precision cut goat liver slices are used as an in-vitro model for the evaluation of  antioxidant activity by craftingin-vivo tissue environment and helps in curtailing the use of intact animals. Plant derived products have been engrossed for the therapy of oxidative stress associated pathological diseases. One such phytocompound is Arjunolic acid, a terterpinoidalsaponin from Terminaliaarjuna bark. The present study is focused to evaluate the antioxidant potential of Arjunolic acid in an in-vitro system of goat liver subjected to oxidative stress. The liver slices treated with hydrogen peroxide (untreated control group) alone showed significant decrease in both enzymatic and non-enzymatic antioxidant levels as compared with normal liver slices. The Arjunolic acid treated liver slices showed significant improvement in both enzymatic and non-enzymatic antioxidant levels as compared with untreated control group. From our results it was observed that Arjunolic acid showed significant antioxidant activity in an in-vitro system of goat liver slices subjected to oxidative stress.