TY - JOUR AU - P. Sai Sharanya, AU - S. Shoba Rani, PY - 2022/01/02 Y2 - 2024/03/28 TI - Development and Validation of Stability Indicating Analytical Method for the Simultaneous Estimation of Netarsudil and Latanoprost by RP-HPLC: https://doi.org/10.54037/WJPS.2022.100111 JF - World Journal of Pharmaceutical Sciences JA - World J Pharm Sci VL - 10 IS - 01 SE - Research Article DO - UR - https://wjpsonline.com/index.php/wjps/article/view/stability-indicating-estimation-netarsudil-latanoprost-hplc SP - 104-112 AB - <p>A simple, Accurate, Precise method was developed for the simultaneous estimation of the Netarsudil and Latanoprost in opthalmic solution dosage form. Chromatogram was run through Zodiacsil C18 150 x 4.6 mm, 5ยต. Mobile phase containing 0.01N Ammonium acetate: Methanol taken with in the ratio 55:45 was pumped through column on a flow rate at 1.0ml/min. Buffer used in this method was Ammonium acetate. Temperature was maintained at 30<sup>o</sup>C. Optimized wavelength selected was 225nm. Retention time of Netarsudil and Latanoprost were found to be 2.222 min and 2.706 min. %RSD of the Netarsudil and Latanoprost were and found to be 0.5 and 0.8 respectively. %Recovery was obtained as 100.09% and 100.5% for Netarsudil and Latanoprost respectively. LOD, LOQ values obtained from regression equations of Netarsudil and Latanoprost were 0.07, 0.22 and 0.03, 0.09 respectively. Regression equation of Netarsudil y = 33253x +578.8 and y =45497x + 399.2 of Latanoprost. Retention times were decreased and that runtime was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.</p> ER -