World Journal of Pharmaceutical Sciences

ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF REMOGLIFLOZIN AND TENELIGLIPTIN IN PHARMACEUTICAL DOSAGE FORMS BY HPLC

Gogikar Pooja — Thu, 26 Dec 2024 00:00:00 +0000

Remogliflozin and Teneligliptin was estimated with HPLC in a tablet dosage form, it was processed with Discovery C 18 column of dimensions 150 x 4.6 mm and 5μm. ammonium acetate and Acetonitrile was used as MP in it with a ratio of 65:35. Wavelength was identified at 210 nm. Rt of Remogliflozin and Teneligliptin was found at 2.206 and 2.646 min. %RSD of the drugs found at 0.6 % and 0.6%, with that the regression of both was seen at y = 52765x + 1014.4 and y = 73315x + 624.91 respectively, and the mean recovery was 99.70% and 99.93%. All the other paraments were validated and were observed within the limits.

FORMULATION OF NOVEL NANO TRANSDERMAL USING EFFECTIVE COMBINATION OF ACYCLOVIR AND OMEPRAZOLE FOR ENHANCED ANTI-VIRAL ACTIVITY

Dr. Nansri saha — Fri, 03 Jan 2025 00:00:00 +0000

The current review exhibited that Acyclovir and Omeprazole nanogel were effectively evolved by dissolvable dispersion technique .pH was resolved different definition F1-F9 in that F9 have reasonable for gel planning. Drug not entirely set in stone by UV-spectroscopic technique. The arranged nanogel was obscure, with next to no knots, molecule and totals. In this way, every one of the definitions are homogenous. Spreadability measurement concentrate on F9 shown the nanogel is having great Spreadability. Nanogel plans shown consistency territory from 3268-3528 cps. It reasoned that they are steady in nature. In-vitro disintegration study was performed and showed that F9 have great disintegration rate. The molecule size, PDI and zeta potential to figure out the F9 plan. The molecule size, PDI and zeta potential was viewed as in 687.4, 0.842 and -43.7 separately. TEM picture was affirmed the state of round and smooth surface of particles at range 650 nm. Contrasting F9 nanogel definition and acyclovir advertised detailing (MF) by in-vitro discharge study. As per result planned Acyclovir and omeprazole nanogel is more effective than the promoted acyclovir salve. Subsequently from our review the acyclovir and omeprazole nanogel (F9) showed that support drug discharge than the showcased plan, so it is obvious that figuring out into nanogel results increment the counter - viral movement.

ANALYTICAL METHOD DEVELOPMENT VALIDATION FOR SIMULTANEOUS ESTIMATION OF PACLITAXEL, GEMCITABINE IN PH DOSAGE FORM BY HPLC

Dr. Sunitha — Thu, 26 Dec 2024 00:00:00 +0000

Gemcitabine and Paclitaxel method validation was estimated by using Agilent column of dimension 150 x 4.6 mm, OPA: MeCN was selected as Mp in 55:45 v/v ratio. This combination was optimized at a spectrum of 256 nm. The Retention of this drugs were eluted at 2.205 and 3.080 for Gemcitabine and Paclitaxel, its RSD was 0.3 and 0.5 with in limit. And the regression was obtained at is y = 89619x + 2823.2, and y = 88164x + 5630.1 respectively. Mean recovery was achieved at 99.20% and 99.50% for Gemcitabine and Paclitaxel respectively and this method was specific with any interference of other drug peak.

METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS ESTIMATION OF MONTELUKAST AND BILASTINE IN PHARMACEUTICAL DOSAGE FORM BY RP-HPLC

Dr. Ch Partiban — Thu, 26 Dec 2024 00:00:00 +0000

By using Agilent (250mm 4.6mm, 5μm) simultaneous estimation of the Bilastine and Montelukast in Tablet dosage form was established. Mobile phase containing 0.1% OPA and CH3CN in 60:40 v/v at a flow of 1.0 ml/min. Temperature maintained at 30°C. Optimized wavelength was 214nm. Retention time of 2.146 min and 3.259 min were found to be Bilastine and Montelukast. %RSD of the Bilastine and Montelukast were and found to be 0.4 and 1.0 respectively. %Recover was 99.85% and 99.98% for Bilastine and Montelukast. LOD, LOQ values were obtained from regression equations of Bilastine and Montelukast were 1.48, 4.47 and 0.30, 0.90 respectively. Regression equation of Bilastine is y = 15205x + 5169.6, and of Montelukast is y = 15205x + 5169.6.

ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF TENELIGLIPTIN AND PIOGLITAZONE IN PHARMACEUTICAL DOSAGE FORMS BY HPLC

Dr. K. Vijayasri — Thu, 26 Dec 2024 00:00:00 +0000

By using HPLC Pioglitazone and Teneligliptin was estimated by using a Agilent C18 column with KH2PO4 together with Acetonitrile in ratio of 40:60 at a flow of 0.9ml/min. the ideal wavelength was detected at 275 nm. The rt of Pioglitazone and Teneligliptin was found at 2.370 min and 2.852 min. the System precision’s RSD got at 0.4 and 0.7%. linearity conc was observed at 7.5-45μg/ml for Pioglitazone and for Teneligliptin was 10-60 μg/ml. the regression from it obtained was y = 21032x + 2298.6 and y = 19667x + 3217 respectively. Our confirmation and observation of all the Other factors were determined while staying within the limits that were defined.

ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF NIVOLUMAB AND RELATLIMAB IN PHARMACEUTICALS DOSAGE FORM BY HPLC

Dr. Divya Yada — Thu, 26 Dec 2024 00:00:00 +0000

The pharmaceutical dosage forms of the immunotherapy treatment medicine Nivolumab and Relatlimab were identified using high-performance liquid chromatography (HPLC). A 150 x 4.6 mm Agilent C 18 column with a particle size of 5μm was utilized. Sixty percent ammonium acetate buffer and forty percent acetonitrile make up the mobile phase. A wavelength of 221 nm was identified. Nivolumab had a retention of 2.245 minutes and Relatlimab of 2.736 minutes. The relative standard deviations of the two medications were 0.5% and 0.3%, respectively; the regression lines for the two drugs were y = 76535x + 17268 and y = 78171x + 5761.7, and the Assay results for each drug were 99.64% and 99.91%, respectively. All the other metrics were verified and monitored within the specified ranges

NEW STABILITY INDICATING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY METHOD FOR THE DETERMINATION OF NIRMATRELVIR AND RITONAVIR IN BULK AND TABLET DOSAGE FORM

Arthi — Thu, 26 Dec 2024 00:00:00 +0000

Nirmatrelvir and Ritonavir are prescribed to treat COVID-19. An cheap HPLC approach was developed and is utilized for validation. KH2PO4 and MeCN are combined in an Agilent C18 150x4.6mm, 5m column at a flow rate of 1 ml/min at a temperature of 30 oC using a 60:40 v/v ratio. Its length was 215.0 nm. Nirmatrelvir had a holding time of 2.243 hours, whereas Ritonavir took 2.815 hours. It was discovered that the %RSD was 0.5% and 0.5%. The Nirmatrelvir regression equation was: y = 58409x + 2794. It was also discovered that ritonavir was y = 59191x + 774.23. Additionally, the LOD and LOQ values were discovered, and all tests passed and met ICH criteria.

METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS ESTIMATION OF MEMANTINE AND DONEPEZIL IN PHARMACEUTICAL DOSAGE FORM BY RP-HPLC

Dr. Sunitha — Thu, 26 Dec 2024 00:00:00 +0000

For the reason of evaluating memantine and donepezil in tablet dose form simultaneously, a simple, accurate, and precise technique was established. An Agilent 150mm 4.6mm 5μ filter was used to perform a chromatogram. A mobile phase comprising phosphate buffer and acetonitrile in a ratio of 58:42 with flow rate of 1.0 ml/min. A constant 30°C was maintained. The ideal wavelength for memantine and donepezil was 294 nm. Memantine's and donepezil's retention times were discovered to be 2.190 and 2.968 minutes, respectively. The percentage RSD for memantine, donepezil was determined to be 0.9 and 0.3, respectively. For memantine and donepezil, recovery was obtained at 99.94% and 99.84%, respectively. Regression models for memantine and donepezil yielded LOD and LOQ values of 0.07 ppm, 0.20 ppm and 0.01 ppm, 0.04 ppm, respectively. The regression equations for memantine (y = 9960.2x + 51.857) and donepezil (y = 9514.8x + 793.79)

PREPARATION AND EVALUATION OF RIFAMPICIN - ASCORBIC ACID LOADED PLGA NANOPARTICLES

Dr. Nansri saha — Thu, 26 Dec 2024 00:00:00 +0000

The point of the current paintings turned into to restriction or stop the debasement of rifampicin, the antitubercular drug in gastric pH situation to work at the power and helpful adequacy of the drugs. The evaluate become carried out via getting equipped Rifampicin stacked PLGA nanoparticles using ascorbic corrosive as a cellular reinforcement. Dug stacked nanoparticles were synthetic by a multistep emulsion approach and assessments of the arranged nanoparticles were then completed through special techniques. In this examine 4 kinds of information have been geared up. Definition 1 (F1) is rifampicin alone stacked PLGA nanoparticles, detailing II (F2) is rifampicin - ascorbic corrosive (1:1) stacked PLGA nanoparticles, plan III (F3) is rifampicin - ascorbic corrosive (1:2) stacked PLGA nanoparticles and plan IV (F4) is rifampicin - ascorbic corrosive (1:3) stacked PLGA nanoparticles. The evaluation presumed that ascorbic corrosive can restriction the corruption of rifampicin in acidic pH circumstance and on this way works at the dependability and bioavailability of rifampicin. The results likewise show that there is a measurably huge alternate inside the fee drug debasement profile while the centralization of ascorbic corrosive changed into multiplied.