In vitro cytotoxic activity of silver nano particle biosynthesized from Colpomenia sinuosa and Halymenia poryphyroides using DLA and EAC cell lines

Abstract

This study was conducted to investigate the invitro cytotoxic activity of silver nano particles biosynthesized from Colpomenia sinuosa and Halymenia poryphyroides using DLA and EAC cell lines by tryphan blue dye exclusion technique and MTT assay using Mouse L929 cell lines (Lungs fibroblast). The results of the trypan blue dye exclusion assay indicates that the silver nano particles biosynthesized from Colpomenia sinuosa and Halymenia poryphyroides inhibits the growth of DLA and EAC cell lines in a dose dependent manner against the standard drug Curcumin where the silver nano particle biosynthesized from Colpomenia sinuosa showed 61.57 % and silver nano particle biosynthesized from Halymenia poryphyroides showed 89.36 % in DLA cell line similarly the silver nano particle biosynthesized from Colpomenia sinuosa showed 81.96 % and silver nano particle biosynthesized from Halymenia poryphyroides 91.45 % in EAC cell line. The results of the MTT assay indicated the silver nano particles biosynthesized from Colpomenia sinuosa and Halymenia poryphyroides significantly inhibited the proliferation of L929 cells in dose dependent manner where the silver nano particle biosynthesized from Colpomenia sinuosa showed 37.06 % and silver nano particle biosynthesized from Halymenia poryphyroides showed 100 % against the standard drug Curcumin.