Dyslipidemia and antioxidant properties of Lycopene and Vitamin E in alloxan-induced diabetes

Austine E Ighorodje; Adaku V Iwueke; Kelechi U Akataobi; Vitalis O Ogbonna; Comfort C Monago-Ighorodje

Authors

Austine E Ighorodje Department of Clinical Pharmacy and Pharmacy Administration, Obafemi Awolowo University, Ile-Ife
Adaku V Iwueke Biochemistry Unit, Department of Science Laboratory Technology Imo State Polytechnic, Umuagwo-Owerri
Kelechi U Akataobi Department Medical Biochemistry, College of Medicine, Abia State University, Uturu
Vitalis O Ogbonna Department of Biochemistry, Madonna University, Elele
Comfort C Monago-Ighorodje Department of Biochemistry, University of Port Harcourt, Choba, Port Harcourt

Keywords:

Antioxidant, Diabetes mellitus, Dyslipidemia, Lycopene, Triacyglycerol, Vitamin E

Abstract

Cardiovascular diseases implicated in Diabetes mellitus include diabetic dyslipidemia consisting of low high density lipoprotein, increased triglycerides and postprandial lipidemia. This is resulting from high level of glucose or hyperglycemia leading to oxidative stress via glucose auto-oxidation, damaging various organs including the liver. The aim of this study is to evaluate the dyslipidemia and antioxidant properties of Lycopene and Vitamin E in the management of diabetes and its associated lipidemia. The experimental Albino rats used were divided into eight groups. The first group served as normal control and was not induced with alloxan, while Groups 2 to 8 were made to develop diabetes with 50mg/kg body weight of rats with alloxan for 72 hours. Group 2 was not treated and served as a negative control while Groups three to eight were treated with three concentrations of Lycopene (80mg, 90mg, 100mg/kg body weight) and Vitamin E (0.1mg,0.2mg, 0.3mg/kg body weight) respectively for four weeks. Blood samples were collected at weekly intervals and analyzed for glucose, triacylglyceride, cholesterol, superoxide dismutase(SOD) and catalase (CAT).The increased level of glucose(mg/dl) was significantly ((p< 0. 05) reduced from the diabetic range of 175.00+6.02-173.67+1.86 to that of 116.05+0.33-115.21+0,04 and 116.16+0.01-114.81+0.02 after treatment with Lycopene (100mg) and Vitamin E (0.3mg).The decreases were significant as the doses were increasing. After induction of diabetes, triacylglycerides and cholesterol were significantly increased showing lipidemia, but were reduced by Lycopene and Vitamin E especially at higher doses. The diabetic level of SOD (132+57.02) and that of CAT (95.33+1.20) after the 4^th week, were reduced by Lycopene and Vitamin E to 24.67 +0.67 and 26.00+0.57, for SOD :24.67+0.76 and 26.00+0.57 for CAT respectively. This work has shown that Lycopene and Vitamin E could be used in management of lipidemia and oxidative stress in diabetes mellitus.