Effect of some elicitors on chemicals composition for Nigella sativa callus cultures

Authors

  • Mona Mohamed Ibrahim Department of Plant Biotechnology, Genetic Engineering and Biotechnology Division, National Research Centre (NRC), Dokki region, Egypt
  • Nermeen Mohamed Arafa Department of Plant Biotechnology, Genetic Engineering and Biotechnology Division, National Research Centre (NRC), Dokki region, Egypt
  • Mohamed Ahmed Matter Department of Plant Biotechnology, Genetic Engineering and Biotechnology Division, National Research Centre (NRC), Dokki region, Egypt

Keywords:

Nigella sativa, Sliver nitrate, Salicylic acid, Fatty acids, Essential oil, GC-MS analysis

Abstract

Callus culture from leaf explants of Nigella sativa was used to study the effect of silver nitrate (AgNO3) and salicylic acid (SA) at 0.5mM as elicitor compounds on the chemical composition of N. sativa for two and four days. Total fatty acids and fatty acid esters increased with AgNO3 addition for two days represent 29.73% of total constituents, while essential oils constituents were enhanced by salicylic acid addition for two days represent 79.31% of total constituents. These elicitors (AgNO3 and SA) proved to be good elicitor compounds to increase secondary metabolites (fatty acids and essential oil) in N. sativa callus culture.    

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Published

2015-10-03

How to Cite

Mona Mohamed Ibrahim, Nermeen Mohamed Arafa, & Mohamed Ahmed Matter. (2015). Effect of some elicitors on chemicals composition for Nigella sativa callus cultures. World Journal of Pharmaceutical Sciences, 3(10), 2160–2166. Retrieved from https://wjpsonline.com/index.php/wjps/article/view/elicitors-chemicals-composition-nigella-sativa-callus-cultures

Issue

World J Pharm Sci 2015; 3(10): 1949-2166

Section

Research Article

License

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.