Evaluation of bonton active granules for anti-osteoporotic activity in ovariectomized rat

Abstract

Osteoporosis is a multi-factorial disease characterized by reduced bone mass and impaired micro-architecture, leading to an increased susceptibility to fractures. The objective of present study was to evaluate anti-osteoporotic activity of Bonton Active Granules in ovariectomized rat model at two different dose levels i.e. Therapeutic Effective Dose (TED) 0.9 g/kg/day and double the dose (TED×2) 1.8 g/kg/day. 24 healthy female wistar rats were divided into 4 groups where each group was containing 6 animals. Group-1 was considered as a normal control group fed with 1% Carboxy Methyl Cellulose (CMC) suspension. Group-2, a Disease control group was containing ovariectomized (OVX) rates fed with 1% CMC suspension. Group 3 and 4 were orally treated with test drug at TED (0.9 g/kg/day) and TED×2 (1.8 g/kg/day) respectively. Present study included parameters like serum alkaline phosphatase (ALP), serum calcium, femoral bone parameters, bone breaking strength, body weight and histopathological study of bone. Treatment with Bonton Active Granules at both dose levels showed significant decrease in serum ALP and significant increase in serum calcium level. It also showed significant changes in femoral parameters and histopathology of bone. Hence, it can be inferred that Bonton Active Granules at both experimented therapeutic doses provides good anti-osteoporotic activity against ovariectomized rat.