Genotyping of HLA Class II Alleles Associated with Some Immunological Markers in Patients with Hydatidosis

Abstract

Hydatidosis is a worldwide parasitic disease which results in serious health problem and financial loss. Different alleles Human leukocyte antigen (HLA) were found to be associated with the susceptibility to different diseases. The current study aimed to determine the impact of different HLA alleles on the susceptibility to cystic echinococcosis (CE) and on serum levels of some immunological markers. The study involved 30 patients with CE and 20 healthy controls. DNA was extracted from a blood sample taken from each subject. HLA class II gene was amplified using Sequence-specific oligonucleotide primed PCR (SSO-PCR). Molecular typing of HLA alleles DR and DQ was performed using a reverse hybridization Automatic Line probe assay (Auto-LiPA).  Enzyme linked immunosorbent assay was used to estimate serum levels of IL-10, TNF-α, IgG4 and IgE in HC-patients and control. There was significant increase in the frequency of HLA-DR*0403 and*0701 in healthy control and patients respectively. Furthermore, significant increase in serum levels of IL-10, IL-4, IgE and IgG4 in hydatidosis patients compared to healthy control was recorded. Thus it cab concluded that alleles HLA-DR*0701 and HLA-DR*0403 are associated with susceptibility and resistance respectively to hydatidosis in Iraqi population.