Stability indicating HPLC method for simultaneous estimation of hydrochlorothioazide, amlodipine and valsartan in pharmaceutical dosage form

Dondeti Mogili Reddy; Putchakayala Purnachandra Rao; D. Ramachandran

Stability indicating HPLC method for simultaneous estimation of hydrochlorothioazide, amlodipine and valsartan in pharmaceutical dosage form

Authors

Dondeti Mogili Reddy Department of Chemistry, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur, Andhra Pradesh, India-522510
Putchakayala Purnachandra Rao Department of Chemistry, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur, Andhra Pradesh, India-522510
D. Ramachandran Department of Chemistry, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur, Andhra Pradesh, India-522510

Keywords:

HPLC, Hydrochlorothioazide, Amlodipine and valsartan

Abstract

A new HPLC method was developed and validated for the determination of HCTZ, Amlodipine, Valsartan in tablet dosage form. The chromatographic separation was achieved on a Inertsil ODS 3V(4.0 X 100mm, 5m) with a mobile phase combination of 0.1% TFA buffer and Acetonitrile (gradient) at a flow rate of 1.0 ml/min, and the detection was carried out by using UV detector at 251nm. The total run time was 10 minutes. The retention time of HCTZ, Amlodipine, Valsartan were found to be 1.58 min., 3.28min., and 5.65 min. respectively. The performance of the method was validated according to the present ICH guidelines.

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Published

2014-12-01

How to Cite

Dondeti Mogili Reddy, Putchakayala Purnachandra Rao, & D. Ramachandran. (2014). Stability indicating HPLC method for simultaneous estimation of hydrochlorothioazide, amlodipine and valsartan in pharmaceutical dosage form . World Journal of Pharmaceutical Sciences, 2(12), 1830–1836. Retrieved from https://wjpsonline.com/index.php/wjps/article/view/hplc-simultaneous-hydrochlorothioazide-amlodipine-valsartan