HPTLC finger printing profile of flower extract of Punica granatum

Authors

  • R. Nalini Research Scholar, PG And Research Department of Biochemistry, Sengamala Thayaar Educational Trust Women’s College, Mannargudi, Tamilnadu, India -614001.
  • R. Anuradha PG and Research Department of Biochemistry, Sengamala Thayaar Educational Trust Women’s College, Mannargudi, Tamilnadu, India - 614001.
  • S. Kamalaveni PG and Research Department of Biochemistry, Sengamala Thayaar Educational Trust Women’s College, Mannargudi, Tamilnadu, India - 614001.

Keywords:

Punica granatum, HPTLC Fingerprint, Phytoconstituent, Quercetin

Abstract

The main objective of the study is to investigate the fingerprint profile of Punic granatum using high performance thin layer chromatography (HPTLC) technique. Camag HPTLC system equipped with TLC Linomat V applicator, Camag TLC scanner III and winCATS software were used. Hydroalcoholic extracts of the flower of Punic granatum was developed in suitable mobile phase using standard procedures and the plates were kept in Photo-documentation chamber (CAMAG REPROSTAR 3) and captured the images at UV light at 254 nm and  366 nm. The fluorescent band (under 254 nm and 366 nm) at Rf 0.56 in mobile phase Toluene: Ethyl acetate: Formic acid (5:4:1) was found and the marker compound Quercetin was quantified.

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Published

2017-08-01

How to Cite

R. Nalini, R. Anuradha, & S. Kamalaveni. (2017). HPTLC finger printing profile of flower extract of Punica granatum. World Journal of Pharmaceutical Sciences, 5(8), 136–142. Retrieved from https://wjpsonline.com/index.php/wjps/article/view/hptlc-finger-printing-profile-flower-extract-punica-granatum

Issue

World J Pharm Sci 2017; 5(8): 115-220

Section

Research Article

License

Creative Commons License

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