Isolation and molecular characterization of urinopathogenic associated Klebsiella pneumoniae from urinary tract infected patients

Abstract

Urinary tract infection is a significant cause of morbidity and mortality. An infection occurs when tiny organisms usually bacteria from the digestive tract, cling to the urethra. A total of 120 urine samples were collected from various hospitals around the Erode district of Tamil Nadu. Out of 120 urine samples were studied, growth of bacteria was observed in 34 urine samples and no growth were seen in 86 samples they found to be healthy. The infected patients were found to be the 15 to 37 years old. The urine samples showed gram negative rod shaped bacteria, non-motile and capsulated bacteria. The respective cultural morphology, biochemical tests and carbohydrate fermentation tests it clear that isolated belongs to be Klebsiella pneumoniae. The continuous observation showed that Klebsiella has replaced other predominant urinary pathogens. Antibiotic sensitivity test revealed that Klebsiella pneumoniae was resistant to six antibiotics by clinical and laboratory standards. Resistance organism has high molecular weight of about 5258bp for Klebsiella pneumoniae. Multiple restriction sites of plasmid are analyzed by using EcoR1, Hind III, BamH1 restriction enzymes and found were digested product.