Physico-phytochemical analysis & Estimation of total phenolic, flavonoids and proanthocyanidin content of Persea americana (avocado) seed extracts

Abstract

The Persea americana is a valuable and important fruit, used in folklore medicine and research studied also exhibited that they have many pharmacological activities such as antioxidant, Anti-inflammatory, antihypertensive, hepatoprotective, hypolipidemic, and recently amoebicidal activities and various other biological activities. Maintaining quality standards of drugs is the need of today, because of its increasing use.

The present study was designed to evaluate the standard profile of the seeds of avocado. The extracts obtained by using different solvents. Phytochemical, physiochemical and TLC analysis were then carried out and loss on drying, total ash content, the yield of acid insoluble ash, the yield of alcohol soluble ash, the yield of water soluble ash were found to be 9.28, 2.53, 2.19, 45.26 and 5.79%w/w respectively. Phytochemical analysis like phenols, flavanoids, terpenoids, steroids, alkaloids, saponin, carbohydrates, protein, amino acids, phytosterols were found to be present in different seed extract of Persea americana. TLC system for analysis was shown to be Chloroform:Methanol:Formic acid was used in the ratio of (60:40:2) respectively. The bands found in this method and its Rf values 0.86cm indicate the presence of β –carotene (0.92cm), which is a tetraterpenoid. The results significantly validate the use of Persea americana and help to many researchers about quality and purity of avocado seeds.