Protease Inhibitor Expression with Immobilized Cells of Egyptian Streptomyces lavendulae on Different Radiated Matrices Using Gamma Radiation

Abstract

Immobilization technique using different matrix (agar, polyacrylamide and gelatin) to enhancement protease inhibitor activity and specific activity from Streptomyces lavendulae (Egyptian isolate from marine) which identified by 16SrRNA with accession number AB795940. The best specific activity of protease inhibitor using numerous factors plus UV radiation was 1055 u/mg however the specific activity without any factor 234 u/mg. Comparing free cells data with immobilization data great different observed, specific activity of protease inhibitor when Streptomyces lavendulae immobilized on gelatin after three days less cell leakage 0.7 and specific activity was 660 u/mg. when gelatin matrix exposure different doses of gamma radiation  then  immobilized bacterial cells specific activity reached to 1900 u/mg  at 8 KGy. The gelatin immobilized cells of Streptomyces lavendulae can be proposed as an effective biocatalyst for repeated usage for maximum production of protease inhibitor.