The relationship among mitochondrial dysfunction and antibiotic activities on Toxoplasma gondii in vitro

Authors

  • Laís Pessanha de Carvalho Laboratory of Tissue and Cell Biology, State University of North fluminense – Darcy Ribeiro, Campos dos Goytacazes, 28013-602, Rio de Janeiro, Brazil
  • Hellen Jannisy Vieira Beiral Laboratory of Tissue and Cell Biology, State University of North fluminense – Darcy Ribeiro, Campos dos Goytacazes, 28013-602, Rio de Janeiro, Brazil
  • Edésio José Tenório de Melo Laboratory of Tissue and Cell Biology, State University of North fluminense – Darcy Ribeiro, Campos dos Goytacazes, 28013-602, Rio de Janeiro, Brazil

Keywords:

Camptothecin, ciprofloxacin, mitochondria, parasitophorous vacuole, Rhodamine 123, tetracycline, Toxoplasma gondii

Abstract

Toxoplasma gondii is the causative agent of toxoplasmosis, which current chemotherapies are self-limiting due to inability to eliminate the parasite and induce toxic effects. Then, new therapies are necessary and antibiotics in combinations with new drugs can be an alternative. Camptothecin, ciprofloxacin and tetracycline, known by inducing loss of mitochondrial activity, were used to verify their anti-T. gondii activities. Infected host cells were incubated with the antibiotics at different concentrations (1-100 mM) for 12 and 24 h. The parasites were eliminated from 5 mM, 12 h, with no toxic effect to host cell until 50 mM, 24 h. The reversibility assays confirmed that the camptothecin and tetracycline arrested the parasite replicative cycle, but not ciprofloxacin. The mitochondrial activity of parasite were gradually decreased during antibiotic treatments, although no morphological change was observed. In summary, the intracellular tachyzoites were eliminated from intracellular environment in presence of the antibiotics, possibly due to loss of its mitochondrial activity.

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Published

2017-09-02

How to Cite

Laís Pessanha de Carvalho, Hellen Jannisy Vieira Beiral, & Edésio José Tenório de Melo. (2017). The relationship among mitochondrial dysfunction and antibiotic activities on Toxoplasma gondii in vitro. World Journal of Pharmaceutical Sciences, 5(9), 225–236. Retrieved from https://wjpsonline.com/index.php/wjps/article/view/relationship-mitochondrial-dysfunction-antibiotic-toxoplasma

Issue

Section

Research Article