Method development and validation for simultaneous estimation of rilpivirine and doultegravir by using RP – HPLC method in bulk dosage form

Authors

  • R. Bhavani Department of Pharmaceutical Analysis, CMR College of Pharmacy, Kandlakoya (V), Medchal Road, Hyderabad, Telangana, India
  • T. Ram Mohan Reddy Department of Pharmaceutical Analysis, CMR College of Pharmacy, Kandlakoya (V), Medchal Road, Hyderabad, Telangana, India

Keywords:

Rilpivirine, HPLC, ICH Guidelines

Abstract

A simple, Accurate, precise method was developed for the simultaneous estimation of the Dolutegravir and Rilpivirine in Tablet dosage form. Chromatogram was run through Agilent C18 (4.6 x 150mm, 5µm) Mobile phase containing Buffer 50% OPA: 50% Acetonitrile   was pumped through column at a flow rate of 1 ml/min. Temperature was maintained at 30°C. Optimized wavelength selected was 257 nm. Retention time of Dolutegravir and Rilpivirine were found to be 2.399 min and 2.853 min. %RSD of the Dolutegravir and Rilpivirine were and found to be 0.8 and 1.6 respectively. %Recovery was obtained as 99.06% and 100.16% for Dolutegravir and Rilpivirine respectively. LOD, LOQ values obtained from regression equations of Dolutegravir and Rilpivirine were 1.08, 3.26 and 0.56, 1.69 respectively. Regression equation of Dolutegravir is y = 11916x + 4431., and y =208758x + 2773. of Rilpivirine. Retention times were decreased and that run time was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.

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Published

2020-12-05

How to Cite

R. Bhavani, & T. Ram Mohan Reddy. (2020). Method development and validation for simultaneous estimation of rilpivirine and doultegravir by using RP – HPLC method in bulk dosage form. World Journal of Pharmaceutical Sciences, 8(12), 167–175. Retrieved from https://wjpsonline.com/index.php/wjps/article/view/simultaneous-estimation-rilpivirine-doultegravir-hplc

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Section

Research Article