New Simple UV Spectrophotometric Method for Determination of Certain NSAIDs present in a Physical Mixture with Pantoprazole in Different pH Values

Abstract

Non-steroidal anti-inflammatory drugs are among the most commonly prescribed agents for rheumatic disorders such as osteoarthritis, rheumatoid arthritis and ankylosing spondylitis. We review the co-prescription of proton pump inhibitors for the prevention of NSAID-induced gastropathy. Ketorolac/ pantoprazole formula and diclofenac/ pantoprazole formula were been studied. An accurate simple and précised method was adopted for simultaneous determination of ketorolac and pantoprazole in a physical mixture form. The method is based on measuring the first derivative amplitudes at 285.2nm and 270.9nm for ketorolac and pantoprazole respectively in 0.1NHCl using 0.1NHCl as a blank. The first derivative values of absorbance at 340nm and 227nm were measured for ketorolac and pantoprazole respectively in phosphate buffer (pH7.4) using phosphate buffer (pH7.4) as a blank. The first derivative values of absorbance at 285nm and 272.6nm for diclofenac and pantoprazole respectively in 0.1NHCl using 0.1NHCl as a blank. The first derivative values of absorbance at 288.9nm and 275.6nm were measured for diclofenac and pantoprazole respectively in phosphate buffer (pH7.4) using phosphate buffer (pH7.4) as a blank. The obtained results were validated for accuracy, precision, LOD, LOQ and were found to be satisfactory. The proposed method is sample, rapid and suitable for the assay of such combinations.