Stability indicating RP-HPLC method development and validation for the simultaneous estimation of vilanterol and umeclidinium bromide in bulk and pharmaceutical dosage forms
Keywords:Vilanterol, Umeclidinium bromide, RP-HPLC
A simple, Accurate, precise method was developed for the simultaneous estimation of the Vilanterol and Umeclidinium bromide in dosage form. Chromatogram was run through BDS C18 (150 x 4.6 mm, 5.0m). Mobile phase containing Buffer 0.1% Formic acid: Acetonitrile taken in the ratio 65:35 was pumped through column at a flow rate of 1.0ml/min. Temperature was maintained at 30°C. Optimized wavelength selected was 265 nm. Retention time of Umeclidinium and Vilanterol were found to be 2.363 min and 3.101 min. %RSD of the Vilanterol and Umeclidinium bromide were and found to be 0.4and0.7respectively. %Recovery was obtained as 99.49%and 101.14% for Vilanterol and Umeclidinium bromide respectively. LOD, LOQ values obtained from regression equations of Vilanterol and Umeclidinium bromide were 0.19, 0.56 and 0.59, 1.8 respectively. Regression equation of Vilanterol is y = 69945x + 7045 and y = 89939x + 60718 of Umeclidinium bromide. Retention times were decreased and that runtime was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.
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