Therapeutic potential and structural elucidation of a water-soluble polysaccharide of a wild edible mushroom Agaricus bisporus against neurodegenerative disease, Alzheimer

Authors

  • Manal G. Mahmoud Microbial Biotechnology Department, National Research Center, Dokki, Cairo, Egypt
  • Abeer Y. Ibrahim Medicinal and Aromatic Plants Researches Department, National Research Centre, Dokki, Cairo, Egypt
  • Mohsen M. S. Asker Microbial Biotechnology Department, National Research Center, Dokki, Cairo, Egypt
  • Osama H. El Sayed Microbial Biotechnology Department, National Research Center, Dokki, Cairo, Egypt

Keywords:

Agaricus bisporus, soluble polysaccharides, structural elucidation, antioxidant, Alzheimer

Abstract

Water-soluble polysaccharide (AB) is soluble polysaccharides extracted by hot water from Agaricus bisporus. Its composition was analyzed by HPLC, IR spectroscopy, and gel permeation chromatography (GPC). The results indicated that AB was composed of glucose and galactose in molar ratio of 6:1 with molecular weight of 19.4 KDa.  AB showed potent antioxidant effect in an in-vitro assay. It was powerful as radical scavenger, reducing agent, metal chelator and inhibitor for lipid peroxidation. AB has been tested on Alzheimer's disease (AD) in particular on its antioxidant potential and on AD brain in AlCl3 modal. Rats were divided into four groups (n=15), control, AlCl3 intoxicant treated with AB. Brain tissue was separated for enzymes assays including superoxide dismutase (SOD), catalase (CAT), TBARS level as an index of malondialdehyde (MDA), cholinergic markers and apoptotic. AB treated AD ALCl3 intoxication of CAT and SOD activity, total antioxidant capacity and anti-apoptotic factor (Bcl-2 and BDNF) with enhancing cholenergic biomarker acetylcholine concentration and inhibited acetylcholine esterase activity however it reduced oxidative stress biomarkers (malondialdehyde, hydrogen peroxide and nitric oxide).

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Published

2014-10-01

How to Cite

Manal G. Mahmoud, Abeer Y. Ibrahim, Mohsen M. S. Asker, & Osama H. El Sayed. (2014). Therapeutic potential and structural elucidation of a water-soluble polysaccharide of a wild edible mushroom Agaricus bisporus against neurodegenerative disease, Alzheimer. World Journal of Pharmaceutical Sciences, 2(10), 1136–1145. Retrieved from https://wjpsonline.com/index.php/wjps/article/view/structural-elucidation-water-soluble-polysaccharide-alzheimer

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Section

Research Article