Analytical method development and validation for the simultaneous estimation of Darunavir and Ritonavir by RP-HPLC method

https://doi.org/10.54037/WJPS.2022.100103

Authors

  • Pasala Lydia Grace M. Pharmacy Scholar, Department of Pharmaceutical Analysis, Malla Reddy College of Pharmacy, Maisammaguda, Dhulapally, Hyderabad, Telangana-500014
  • C. Parthiban Professor and HOD, Department of Pharmaceutical Analysis, Malla Reddy College of Pharmacy, Maisammaguda, Dhulapally, Hyderabad, Telangana-500014

Keywords:

Darunavir, Ritonavir, RP-HPLC

Abstract

A simple, Accurate, precise method was developed for the simultaneous estimation of the Darunavir and Ritonavir in tablet dosage form. Chromatogram was run through standard symmetry C18 (4.6 x 150 mm, 5mm). Mobile phase containing Buffer 0.01N KH2PO4: Acetonitrile taken in the ratio 45:55%v/v was pumped through column at a flow rate of 1ml/min. Optimized wavelength selected was 290 nm. Retention time of Darunavir and Ritonavir were found to be 2.131 min and 2.593 min. %RSD of the Darunavir and Ritonavir were and found to be 0.8 and 0.6 respectively. %Recovery was obtained as 99.59% and 99.94% for Darunavir and Ritonavir respectively. LOD, LOQ values obtained from regression equations of Darunavir and Ritonavir were 0.86, 2.60 and 0.09, 0.29 respectively. Regression equation of Darunavir is y = 12533x + 10387 and y = 9061x + 183.8 of Ritonavir. Retention times were decreased and that run time was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.

Downloads

Published

2022-01-02

How to Cite

Pasala Lydia Grace, & C. Parthiban. (2022). Analytical method development and validation for the simultaneous estimation of Darunavir and Ritonavir by RP-HPLC method: https://doi.org/10.54037/WJPS.2022.100103. World Journal of Pharmaceutical Sciences, 10(01), 32–40. Retrieved from http://wjpsonline.com/index.php/wjps/article/view/hplc-estimation-darunavir-ritonavir

Issue

Section

Research Article