Analytical method development and validation for the simultaneous estimation of Darunavir and Ritonavir by RP-HPLC method

https://doi.org/10.54037/WJPS.2022.100103

Authors

  • Pasala Lydia Grace M. Pharmacy Scholar, Department of Pharmaceutical Analysis, Malla Reddy College of Pharmacy, Maisammaguda, Dhulapally, Hyderabad, Telangana-500014
  • C. Parthiban Professor and HOD, Department of Pharmaceutical Analysis, Malla Reddy College of Pharmacy, Maisammaguda, Dhulapally, Hyderabad, Telangana-500014

Keywords:

Darunavir, Ritonavir, RP-HPLC

Abstract

A simple, Accurate, precise method was developed for the simultaneous estimation of the Darunavir and Ritonavir in tablet dosage form. Chromatogram was run through standard symmetry C18 (4.6 x 150 mm, 5mm). Mobile phase containing Buffer 0.01N KH2PO4: Acetonitrile taken in the ratio 45:55%v/v was pumped through column at a flow rate of 1ml/min. Optimized wavelength selected was 290 nm. Retention time of Darunavir and Ritonavir were found to be 2.131 min and 2.593 min. %RSD of the Darunavir and Ritonavir were and found to be 0.8 and 0.6 respectively. %Recovery was obtained as 99.59% and 99.94% for Darunavir and Ritonavir respectively. LOD, LOQ values obtained from regression equations of Darunavir and Ritonavir were 0.86, 2.60 and 0.09, 0.29 respectively. Regression equation of Darunavir is y = 12533x + 10387 and y = 9061x + 183.8 of Ritonavir. Retention times were decreased and that run time was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.

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Published

2022-01-02

How to Cite

Pasala Lydia Grace, & C. Parthiban. (2022). Analytical method development and validation for the simultaneous estimation of Darunavir and Ritonavir by RP-HPLC method: https://doi.org/10.54037/WJPS.2022.100103. World Journal of Pharmaceutical Sciences, 10(01), 32–40. Retrieved from https://wjpsonline.com/index.php/wjps/article/view/hplc-estimation-darunavir-ritonavir

Issue

Section

Research Article