Stability indicating UPLC method for simultaneous estimation of Abacavir, Lamivudine and Dolutegravir from its tablet dosage form

Abstract

A novel stability‐indicating Ultra performance liquid chromatography (UPLC) method has been developed and validated for the simultaneous estimation of Lamivudine, Abacavir and Dolutegravir in the tablet dosage form. Chromatographic separations were carried using Inspire (50 mm x 2.1 mm) 1.8 μm column with a mobile phase composition of phosphate buffer (pH 6) and Acetonitrile in the gradient program have been delivered at a flow rate of 0.3ml/min and the detection was carried out using UV detector at wavelength 242 nm. The retention time for Lamivudine, Abacavir and Dolutegravir were 0.965, 1.528 and 2.186 minute respectively. The correlation coefficient values in linearity were found to be 0.999 and concentration range 30-150 μg/ml, 60-300 μg/ml and 5-25µg/ml respectively. The results of study showed that the proposed RP‐UPLC method is a simple, accurate, precise, rugged, robust, ultra-fast and reproducible, which may be useful for the routine estimation of abacavir sulphate and lamivudine in pharmaceutical dosage form.