Genotyping of the serotonin transporter gene (SLC6A4) and tumor necrosis factor-alpha in patients with irritable bowel syndrome in Iraq

Abstract

Irritable bowel syndrome is chronic functional gastrointestinal disturbance characterized by abdominal discomfort or pain improves by defecation, and accompanied by alter in bowel habits such as diarrhea or constipation, which cannot be explained by biochemical, structural, or metabolic abnormalities. The current study was conducted to evaluate the role of serotonin transporter gene and tumor necrosis factor-α G308A genes polymorphism in the Irritable bowel syndrome. We have investigated single nucleotide polymorphisms of tumor necrosis factor-α G308Agene and insertion /deletion polymorphism of serotonin transporter gene in 110 subjects. Sixty-five were Irritable bowel syndrome patients while others were apparently healthy individuals used as controls. The frequencies of tumor necrosis factor-α A308A (7.7 vs 8.88%) genotypes and A allele(13.07 vs 17.78%) were higher in control group than Irritable bowel syndrome patients while serotonin transporter gene S/S genotypes was(47.7 vs 26.67%) and S allele (66.92 vs 47.78%) were higher in patient than control groups. AA genotype with tumor necrosis factor-α G308A allele polymorphism and S/S genotype with serotonin transporter gene are mainly expressed among patients with Irritable bowel syndrome and susceptibility with disease might be prospected.