Development and validation of bio-analytical method for the quantitative estimation of rivaroxaban by using UV spectrophotometry

Kodipyaka Sahithi; Pola Ravi Kumar; Yenumula Padmavathi; Nayaka Raghavendra Babu; Damanagari Saranya Reddy; Garige Jaya Sravani; Challoori Spandana

Development and validation of bio-analytical method for the quantitative estimation of rivaroxaban by using UV spectrophotometry

Authors

Kodipyaka Sahithi Department of Pharmaceutical Analysis, G. Pulla Reddy College of Pharmacy, Mehidiptanam, Hyderabad, Telangana-28
Pola Ravi Kumar Department of Pharmaceutical Analysis, G. Pulla Reddy College of Pharmacy, Mehidiptanam, Hyderabad, Telangana-28
Yenumula Padmavathi Department of Pharmaceutical Analysis, G. Pulla Reddy College of Pharmacy, Mehidiptanam, Hyderabad, Telangana-28
Nayaka Raghavendra Babu Department of Pharmaceutical Analysis, G. Pulla Reddy College of Pharmacy, Mehidiptanam, Hyderabad, Telangana-28
Damanagari Saranya Reddy Department of Pharmaceutical Analysis, G. Pulla Reddy College of Pharmacy, Mehidiptanam, Hyderabad, Telangana-28
Garige Jaya Sravani Department of Pharmaceutical Analysis, G. Pulla Reddy College of Pharmacy, Mehidiptanam, Hyderabad, Telangana-28
Challoori Spandana Department of Pharmaceutical Analysis, G. Pulla Reddy College of Pharmacy, Mehidiptanam, Hyderabad, Telangana-28

Keywords:

Rivaroxaban, USFDA guidelines

Abstract

The present study deals with the development and validation of bioanalytical method for the quantitative estimation of rivaroxaban by using UV spectrophotometric technique. Maximum absorption of 250 nm, was selected for the analysis and the drug obeyed Beer-Lambert’s law in the concentration range of 1000-7000ng/ml. Coefficient of correlation for linearity was found to be 0.995. The developed method has been validated for accuracy and precision as per USFDA guidelines. LLOQ, LQC, MQC and HQC values were found to be within the acceptable limits. The developed method was successfully applied for determination of rivaroxaban in spiked rat plasma with good recovery.

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Published

2020-02-01

How to Cite

Kodipyaka Sahithi, Pola Ravi Kumar, Yenumula Padmavathi, Nayaka Raghavendra Babu, Damanagari Saranya Reddy, Garige Jaya Sravani, & Challoori Spandana. (2020). Development and validation of bio-analytical method for the quantitative estimation of rivaroxaban by using UV spectrophotometry. World Journal of Pharmaceutical Sciences, 8(2), 38–43. Retrieved from https://wjpsonline.com/index.php/wjps/article/view/144