METHOD DEVELOPMENT AND VALIDATION OF RP-HPLC FOR SIMULANEOUS ESTIMATION OF BUPROPION AND ZONISAMIDE

Abstract

For the simultaneous estimation of the Bupropion and Zonisamide in Pharmaceutical Dosage Form dosage form, a straightforward, accurate, and precise method was developed. Chromatogram was run through Inertsil -ODS C18(250mm x 4.6 mm, 5 µm). Mobile phase containing Methanol: Water taken in the ratio 45:55 was pumped through column at a flow rate of 1.0 ml/min. Temperature was maintained at 30°C. Optimized wavelength selected was 275nm. Retention time of Bupropion and Zonisamide were found to be 3.226 min and 4.529. %RSD of the Bupropion and Zonisamide were and found to be 0.09 and 0.02 respectively. %Recovery was obtained as 99.97% and 99.57% for Bupropion and Zonisamide respectively. LOD, LOQ values obtained from regression equations of Bupropion and Zonisamide were 0.34, 1.05 and 0.25, 0.77 respectively. Regression equation of Bupropion is y = 10695x + 1351.3, y = 208538x + 57895 of Zonisamide. Because retention times and run times were reduced, the method developed was simple and cost-effective, and it can be used in regular quality control tests in industries.