METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS ESTIMATION OF MONTELUKAST AND BILASTINE IN PHARMACEUTICAL DOSAGE FORM BY RP-HPLC

Abstract

By using Agilent (250mm 4.6mm, 5μm) simultaneous estimation of the Bilastine and Montelukast in Tablet dosage form was established. Mobile phase containing 0.1% OPA and CH3CN in 60:40 v/v at a flow of 1.0 ml/min. Temperature maintained at 30°C. Optimized wavelength was 214nm. Retention time of 2.146 min and 3.259 min were found to be Bilastine and Montelukast. %RSD of the Bilastine and Montelukast were and found to be 0.4 and 1.0 respectively. %Recover was 99.85% and 99.98% for Bilastine and Montelukast. LOD, LOQ values were obtained from regression equations of Bilastine and Montelukast were 1.48, 4.47 and 0.30, 0.90 respectively. Regression equation of Bilastine is y = 15205x + 5169.6, and of Montelukast is y = 15205x + 5169.6.