SIMULTANEOUS ESTIMATION OF THE SULBACTAM AND DURLOBACTAM INBULK AND TABLET DOSAGE FORM BY RP-HPLC

Abstract

A simple, Accurate, precise method was developed for the simultaneous estimation of the Sulbactam and Durlobactam in bulk and Tablet dosage form. Chromatogram was run through phenomenexC18 250 x 4.6 mm, 5m. Mobile phase containing Buffer 0.1% OPA: Acetonotrile taken in the ratio 60:40 was pumped through column at a flow rate of 1 ml/min. Buffer used in this method was 0.1% OPA. Temperature was maintained at 30°C. Optimized wavelength selected was 270 nm. Retention of Sulbactam and Durlobactam were eluted at 2.206 min and 2.871 min. %RSD of the Sulbactam and Durlobactam were and found to be 0.8 and 0.9 respectively. %Recovery was obtained as 100.35% and 100.70% for Sulbactam and Durlobactam respectively. LOD, LOQ values obtained from regression equations of Sulbactam and Durlobactam were 0.63, 1.90 and 0.11, 0.34 respectively. Regression equation of Sulbactam is y = 15617x + 4696.6, and y = 18901x + 2971 of Durlobactam. Retention times were decreased and that run time was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.