ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF TENELIGLIPTIN AND REMOGLIFLOZIN IN PHARMACEUTICAL DOSAGE FORMS BY HPLC.

Abstract

By using HPLC Remogliflozin and Teneligliptin was estimated by using a HSS C18 column of dimension 2.1 x 50mm, 1.8μm With KH2PO4 as buffer together with Acetonitrile in ratio of 40:60 at a flow of 0.2ml/min. the ideal wavelength was detected at 215 nm. The rt of Teneligliptin and Remogliflozin was found at 1.375 min and 1.736 min. the System precision’s RSD got at 1.2 and 0.6%. linearity conc was observed at 1.25-7.5 μg/ml for Teneligliptin and Remogliflozin was 12.5-75 μg/ml. the regression from it obtained was y = 11748x + 329.21and y = 4428.9x + 4002.7 respectively. Our confirmation and observation of all the other factors were determined while staying within the limits that were defined.