In-vitro and in-silico analysis of spirulina extracts against rheumatoid arthritis disease: A therapeutic approach

Krishna Kumar Das; Anjana Bhagat; Lopamudra Singha; Souvagyalaxmi Sahoo

Authors

Krishna Kumar Das Tectona Biotech Resource Center (TBRC), Odisha, Bhubaneswar-751002
Anjana Bhagat Tectona Biotech Resource Center (TBRC), Odisha, Bhubaneswar-751002
Lopamudra Singha Dept. of Plant breeding and Genetics, Assam Agricultural University, Jorhat, Assam
Souvagyalaxmi Sahoo Tectona Biotech Resource Center (TBRC), Odisha, Bhubaneswar-751002, India

Keywords:

Rheumatoid arthritis, S. platensis, S. aureus, E.Coli, FRAP-assay, Spirulina

Abstract

The current investigation of a combinatorial approach of in silico and in vitro which was carried out to identify and explore the role of existing drugs and phytochemical compounds against potential targets of RA. In this study we got 150 genes related with the RA disease. From those 18 genes (proteins) were mostly related to 9 drugs to cure the disease. From the literature survey 8 Phytochemicals like Flavonoids, Alkaloids, Steroids, Lutein, Saponins, Tannin, Lycopene, and Alpha-Carotene were mostly present in Spirulina. These 4 proteins IL2RA, CFLAR, CASP8, and PTPN22 were mainly affected by Alkaloids, Flavonoids, and the Alpha-Carotene. Then the antibacterial activity of S. platensis was evaluated in different concentration of solvent extract (Methanol & Ethyl acetate) against targeted bacterial species (S. aureus and E.Coli). Toxicity of the solvent extract was tested by Paper disc diffusion method. Methanol extract gave the highest zone of inhibition than Ethyl acetate extract for the both bacteria E.Coli and S. aureus. FRAP-assay had been used to determine the antioxidant activity as it was simple and quick and the result shown that the Ethyl acetate extraction was more efficient than the Methanol extraction.