Phytochemical study and anti-radical activity of the hydroethanolic extract of the barks of Erythrophleum suaveolens (Guill. & Perr.) Brenan

T. Andzi Barhé; T. Ngoualali; B. B. Mbourangon; R. P. Ongoka

Authors

T. Andzi Barhé Laboratory of Applied Chemistry Research (LARCA), Ecole Normale Supérieure (ENS), Department of Exact Sciences, University Marien Ngouabi, Brazzaville-CONGO
T. Ngoualali Laboratory of Chemistry of Organic Biomolecules and Pharmacodynamics, National Institute for Research in Health Sciences (IRSSA), Department of Pharmacopoeia and Traditional Medicine, Scientific City of Brazzaville, Congo
B. B. Mbourangon Laboratory of Applied Chemistry Research (LARCA), Ecole Normale Supérieure (ENS), Department of Exact Sciences, University Marien Ngouabi, Brazzaville-CONGO
R. P. Ongoka Laboratory of Applied Chemistry Research (LARCA), Ecole Normale Supérieure (ENS), Department of Exact Sciences, University Marien Ngouabi, Brazzaville-CONGO

Keywords:

Erythrophleum suaveolens, total polyphenols, total flavonoids, Chemical screening, antioxidant activity

Abstract

The article presents the phytochemical study and the evaluation of the anti- radical capacities of the extract of Erythrophleum suaveolens. After chemical screening by colorimetric methods, a dosage of total polyphenols (PPT) and total flavonoids (FVT) by UV-visible spectrometry, the anti-radical activity is evaluated by action with DPPH. The screening of the extract shows the presence of six major groups of chemical compounds: alkaloids, flavonoids, saponosides, tannins, reducing compounds, steroids, and terpenes. The quantitative analysis of flavonoids (FVT) compounds varies between 144.42 and 193.92 mgEQt/100g DM, the amount of total polyphenols varies between 1313.13 and 3075.24 mgEGA/100g DM. This high production of polyphenolic compounds is confirmed by the highly marked anti-radical activity observed on the DPPH radical for the fractions F2 (8.1 μg/ml), F3 (7.2 μg/ml) and F4 (7.2 μg/mL). This activity remains low compared to the standard compounds used, Quercetin (2.7 µg/ml), Gallic Acid (1.6 µg/ml), but justifies the use of this bark in traditional medicine.