STABILITY INDICATING RP-HPLC METHOD FOR ESTIMATION OF BELZUTIFAN IN ITS PHARMACEUTICAL DOSAGE FORM

Abstract

The RP-High Performance approach is straightforward, delicate, precise, and consistent to gauge Belzutifan in drug (forms of administration and Extracts. Used materials in chromatographic techniques like stationary phase is Kromosil (4.6mm x 150mm, 5μm) was applied along with the solvent fissure MeCn and Dipotassium buffer. The instrumental conditions setup after method optimization are warmth setup of 30oC and Solvent fissure at 60 and 40 where buffer has been loaded higher than organic, and effluent velocity maintained at 1.0ml/min and last frequency of lamda seen the analyte absorbance was 251.0nm. The Belzutifan maintenance season was observed to last 2.248 minutes. the Eluite % R was evaluated as 0.8, while the %RSD of its methodology precision was evaluated as 0.9. the fraction of trueness percent for Belzutifan was 101.03%. The detective and quantification values obtained from Regression method were 0.31 and 0.94 and R2 is y = 93023x + 81190. Since the duration of retention and the run period were shortened, the procedure was simple and cost-effective, making it suitable for use in periodic inspection and quality tests in organizations.