M. Pharmacy, Department of Pharmaceutical Quality Assurance, Sultan Ul Uloom College of Pharmacy, Banjara hills, Mount hills, Hyderabad, 500034.

Abstract

Pharmaceutic dose form and bulk form: Carboprost via RP-HPLC Methodology Carboprost's absorption maxima turned out to be 228.0 nm. Agilent C18 150 mm 4.6 mm 5 μm X 100 Å stationary phase Agilent conditions; mobile phase 1N Citrate buffer; methylene chloride and water in a 55:45 ratio. Next pH changed to 3.5. Furthermore, flow rate was kept at 1.1 ml/min; the detection wave length was 228 nm; column temperature was set at 26.0oC; and diluent was mobile phase. Conditions were determined as ideal approach. Carboprost's retention period turned shown to be 2.295 min. The Carboprost's % RSD turned out to be 0.4% correspondingly. %Recovery came up as 99.90% for Carboprost correspondingly. Carboprost's LOD, LOQ values from its regression equations came out as 0.04 and 0.11 respectively. For Carboprost especially, %Assay came out as 99.80%. Carboprost's equation of regression is y = 52769x + 663.92. Retention periods were lowered and run durations were lowered; so, the created approach was easy and cost-effective that one could apply in routine Quality control tests in different industries. Carboprost degradation investigations were conducted with an acceptable range and a greater than purity angle threshold in all conditions.