STABILITY INDICATING RP-HPLC METHOD FOR THE DEVELOPMENT AND VALIDATION OF TIVOZANIB IN BULK AND PHARMACEUTICAL DOSAGE FORMS

Abstract

Tivozanib determination in pharmaceutical dosage form using RP-HPLC technique Run through Kromosil C18 (4.6mm x 150mm, 5μm) the chromatogram. Methanol taken in the 60:40 ratio was pumped through column at a flow rate of 1.0ml/min in mobile phase including 0.1% Perchloric acid. Temperature maintained at thirty-degree Celsius. Selected a wavelength optimised at 230.0 nm. Tivozanib's retention time came up as 2.696min. %RSD of the Tivozanib turned out to be 0.6%. PercentageMethod accuracy of Tivozanib shown to be 0.6% RSD.For Tivozanib, recovery was 100.67%. Regression equation of Tivozanib is y = 52314x + 17011. LOD, LOQ values derived from this equation were 0.08, 0.24. Retention durations were lowered and run time was lowered; so, the new approach was straightforward and cost-effective that one could apply in routine Quality control tests in different industries.