ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR THE ESTIMATION OF CYANOCOBALAMIN INJECTION (B12)

Abstract

A precise RP-HPLC method for the pharmaceutical dose estimation of Cyanocobalamin. Chromatogram was run through Sunfire C18 250 x 4.6 mm, 5m. Mobile phase containing 0.01N Kh2po4: Methanol taken in the ratio 60:40 v/v was pumped through column at a flow rate of 1.0ml/min. Temperature was maintained at 30°C. Optimized wavelength selected was 251 nm. Retention time of Cyanocobalamin was found to be 2.461 min. standard %RSD of the Cyanocobalamin were and found to be 0.6. %RSD of Method precision of Cyanocobalamin was found to be 0.4. %Recovery was obtained as 99.74% for Cyanocobalamin. LOD, LOQ values obtained from regression equation of Cyanocobalamin were 0.01, 0.03. Regression equation of Cyanocobalamin is y = 33995x + 858.82.