Effect of probiotic (lactobacillus& saccaromyces) on the immunological, biochemical and haematological changes of broiler chicken fed on ochratoxicated ration

Abstract

Eightyone day old Hubbard chicks were divided into four equal groups. First group fed on ration containing only ochratoxin A 2.5mg / 1kg, Second Group fed on ration containing probiotic and ochratoxin A 2.5 mg /1kg, third Group fed on ration containing Probiotic only a fourth group remains as a control fed on plain ration. The experiment continued for 45 days. The chicks vaccinated at 7^th day with Hitchner By by ocular route and at 18^th, 28^th day vaccinated by Lasota vaccine via of drinking water. Every week the birds (weighted with calculation of the amount of ration which have been consumed.  Whole blood was collected at 14, 28, and 42 days of age from all groups. The blood serum collected for evaluation of immune response to ND virus vaccine. Liver and kidney function assays were applied and the collected whole blood for differential leucocytic count Immunosuppression was observed in chickens fed diets containing ochratoxin A for 4 weeks, when compared with controls, in which the treated birds showed reduction in total serum protein, lymphocyte precentage weights of the thymus, bursa of Fabricius, and spleen. Urea, creatinine, cholesterol, also alkaline phosphatase, AST, and ALT exhibited a significant increase in ochratoxicated group by comparison to the control group. The addition of probiotic into the ochratoxicated ration improve both the total body weight and organ weight in comparing with the ochratoxicated ration which showed increasing the relative weight of livers and kidney, on the other hand there were a decreasein the relative weights of bursa of Fabricius and spleen. The obtained results concluded that, the addition of probiotic into the Qchratoxicated ration Improve the Immune response to NDvirus vaccine, total body weight and organ weight and also improve most biochemical and hematological parameters.