Novel electrochemical quantification for nepafenac in raw drug, pharmaceutical eye drops and biological fluid

Abstract

This article pronounces differential pulse voltammetric technique for the determination of nepafenac in pharmaceutical dosage form and human serum. The proposed voltammetric technique was established on electro-oxidation of nepafenac at two electrodes, the first electrode is the carbon paste modified with 10% w/w of 1-n-butyl-3-methylpyridinium hexafluorophosphate ion crystal (10%BMH-CPE) and the second electrode is carbon paste modified with 10% w/w of each of multi walled carbon nanotubes and 1-n-butyl-3-methylpyridinium hexafluorophosphate (CMWNT-BMH-CPE).The method was performed in 0.04M B-R electrolyte with pH=6.0. The peak current concentration relationship was linearand the suggested methods were applied successfully to the pharmaceutical dosage form and spiked human serum with good repeatability and reproducibility.