Hepatoprotective effect of ginger extract against the toxicity of 7, 12-dimethylbenz (a)anthracene (DMBA) in albino rats

Doaa A. Ali; Mohamed F. Ismail; Heba A. Badr

Authors

Doaa A. Ali Department of Zoology, Faculty of Science, Mansoura University, Mansoura, Egypt
Mohamed F. Ismail Department of Zoology, Faculty of Science, Mansoura University, Mansoura, Egypt
Heba A. Badr Department of Zoology, Faculty of Science, Mansoura University, Mansoura, Egypt

Keywords:

Ginger, DMBA, Hepatotoxicity, Histology, Histochemistry, PCNA immunohistochemistry

Abstract

The present work was conducted to study the protective effect of ginger extract (GE) against the hepatotoxicity induced by 7, 12-dimethylbenz(a)anthracene (DMBA) in female rats. DMBA treated group showed a highly significant decrease in body weight. However, DMBA+GE treated group displayed a highly significant increase in body weight compared with DMBA treated group. Histologically, the liver of DMBA treated group showed nodule-like structures, hepatic cirrhosis, congestion of blood vessels, intercellular hemorrhage, hepatic pyknotic nuclei, lymphocytic infiltration, dilation of blood sinusoids and large amount of collagen fibres. DMBA+GE treated group displayed an improvement in the hepatic lesions induced by DMBA. Histochemically, the liver sections of DMBA treated group showed a marked depletion of polysaccharides and total protein content. However, DMBA+GE treated group showed a moderate increase in liver polysaccharides and total protein contents. Immunohistochemically, the hepatocytes of DMBA treated group showed a highly significant increase in the PCNA labelling index. However, DMBA+GE treated group displayed a highly significant decrease in the PCNA labelling index when compared with DMBA treated group.