Synthesis, spectroscopic characterization and pharmacological evaluation of novel copper mediated antibiotics

Abstract

A series of novel copper mediated antibiotics were prepared from N⁴ – substituted having structure [Cu (L)₂] Cl₂. All compounds were characterized by various physico -chemical techniques like melting point, TLC, elemental analysis, IR, H¹-NMR, LC-MS, UV-Visible spectroscopy. The magnetic moments and electronic spectral studies suggested distorted octahedral geometry for all the compounds. The monoanionic orthophenylene diammine ligands were in a bidentated mode, binding through azomethine nitrogen atoms of substituted bis carbohydrazones. The synthesized compounds were screened for their in vitro antibacterial activity using disc diffusion method against two strains of gram negative and gram positive bacteria. Streptomycin antibiotic was used as positive control. All compounds showed significant antibacterial activity in the range of 1-10 mg/ml. Invitro antioxidant activity of the all copper compounds was screened by using the DPPH scavenging assay by using ascorbic acid as a positive control. All compounds   exhibited potent antioxidant activity in the range 80-90%. . The Invitro-cytotoxic activity of compounds against breast cancer cell lines MCF-7 was evaluated for all the above compounds. All compounds were found to be highly active against the studied cell line; presenting the similar values of IC₅₀ around 10 mmol/L. These compounds would be evaluated further for their possible DNA binding, cleavage, antifungal and anti-diabetic properties.