Troubleshooting and maintenance of high-performance liquid chromatography- A Review

Abstract

HPLC (High Performance Liquid Chromatography) has evolved into an indispensable tool in many analytical laboratories and is applied to diverse analytical problems. Troubleshooting HPLC instrumentation and separations require a fundamental understanding of how the instrument functions and how the separation works. This review gives an overview of HPLC system maintenance and summarizes strategies and guidelines for HPLC troubleshooting. Every HPLC system consists of the same basic components, no matter if it is a modular system. Problems can take place in each component, can change the overall performance, and will consume more cost to recover the problems. This review paper provides common maintenance procedures that can be performed by the user and simple guidelines for maintenance and troubleshooting of HPLC and solving characteristic and common problems in HPLC. An easy-to-use table describes probable cause and solutions. The objective behind this article to help the Analyst in troubleshooting and maintenance of High Pressure Liquid Chromatography, and to overcome the shortcomings that may arise during their projects.