New, sensitive and validated spectrofluorimetric method for the estimation of desvenlafaxine succinate in bulk and in pharmaceutical formulations

Abstract

A New, simple, sensitive and economical spectrofluorimetric method for estimation of Desvenlafaxine Succinate, a novel SNRI (serotonin - norepinephrine reuptake inhibitor) in bulk and in solid dosage form was developed in the present study. Desvenlafaxine Succinate showed excitation at 274 nm and emission at 305 nm in pH=6 phosphate buffer which was used in this study. The linear regression equations obtained by least square regression method, was Int =0.396*Conc. (in ng/ml) -1.701. The method provides a linear response across a quantization range of 100ng/ml to 900ng/ml. The developed method was found to have high degree of precision and accuracy and was used for the estimation of total drug content in the commercial formulation of Desvenlafaxine Succinate-ER tablets. The results of analysis were treated statistically, as per International Conference on Harmonisation (ICH) guidelines for validation of analytical procedures. The results were found to be accurate, reproducible and free from interferences.