The Study of Frequencies of HLA-DR3 and DR4 Among Type 1 Diabetes Mellitus Patients

Mueen H. Hantoosh; Hammadi A. Al-Hilaly; Ferdous A. Jabir

Authors

Mueen H. Hantoosh Department of Medical Microbiology, College of Medicine, Al-Qadisiyah University, Diwaniya, Iraq
Hammadi A. Al-Hilaly Department of Medical Microbiology, College of Medicine, Al-Qadisiyah University, Diwaniya, Iraq
Ferdous A. Jabir Department of Chemistry, College of Medicine, Al-Qadisiyah University, Diwaniya, Iraq

Keywords:

T1DM, HLA, DR3, DR4, Gene, Alleles, Real-time PCR

Abstract

Type 1 diabetes mellitus (T1DM) is so far thought to be an auto-immune disease. This disease has revealed characteristics regarding predisposing factors. One of the genetic predisposing factors is thought to be HLA-DR3 and DR4 genes. The present study aimed at investigating the frequencies of (Human Leukocyte Antigen) HLA-DR3&DR4 genes in a group of 64 Iraqi patients, in whom the T1DM has been already diagnosed and compared to a group of apparently healthy (control group N= 32) individuals. The Real-time PCR was used for the HLA-genes in all members of the study. On the context of genotyping of HLA- allele, DR3 and DR4 were found to be different in their frequencies significantly among T1DM patients that creating high etiological fraction of 0.404 and 0.707 respectively compared to healthy controls, with odd ratio (OR) 4.61 for DR3 and high OR for DR4 26.73. This allele, occurred in high frequencies of the two genes in T1DM patients, was high significantly different between patients group for DR3 compared to healthy group which 51.6% and 18.8% respectively, and very high significantly different between patients group for DR4 compared to healthy group which 73.4% and 9.4% respectively, and both DR3&DR4 also occurred in high significantly difference which 43.8% and 6.3% respectively in T1DM and healthy control group.