Cytotoxic, anti-inflammatory, analgesic, CNS depressant, antidiarrhoeal activities of the methanolic extract of the Artocarpus Lakoocha leaves

Abstract

The study was carried out to assess the cytotoxic, anti-inflammatory, analgesic, CNS depressant and antidiarrhoeal activities of the Artocarpus lakoocha methanolic leaves extract. In brine shrimp lethality bioassay, the LC₅₀ value of the Artocarpus lakoocha leaves was 2.83±0.11 (µg/ml). The anti-inflammatory effect of the extract was observed significant effect at 1^st, 2^nd and 3^rd hour and 200mg/kg showed 64.90% inhibition which is closed to standard (69.86%). The extract (200 mg/kg) exhibited higher analgesic activity against acetic acid induced pain in mice than 100mg/kg dose extract. At both test doses produced a higher percent of protection that is about similar to reference Indomethacin (10 mg/kg) in formalin test. The extract also expressed significant dose dependent CNS depressant activity at 60min, 90min and 120min in both hole cross and open field test. The extract (100 and 200 mg/kg) reduced the frequency and severity of diarrhea in test animals. The results demonstrated that the Artocarpus lakoocha methanolic leaves extract had the potential phytomedicine value for its significant cytotoxic, anti-inflammatory, analgesic, CNS depressant and antidiarrhoeal activity.