The Study of protective effect of Nordihydroguaiaretic acid (NDGA) on cisplatin induced genotoxicity and nephrotoxicity by attenuating oxidative stress in Swiss albino mice

Abstract

Cisplatin is a magic chemotherapeutic drug, unfortunately its dose dependant dark sides may halt its magic’s. It has major dark sides such as genotoxicity and nephrotoxicity. Cisplatin has a strong power to sire a great amount of free oxidative radicals, which contributes to its dark sides. The emphasis of the present study was to investigate whether the pre-treatment of NDGA had any protective consequence against cisplatin-induced genotoxicity and nephrotoxicity. In this study we evaluated the role of NDGA on cisplatin induced oxidative stress in mice through following parameters like chromosome aberrations, micronucleus assay, lipid peroxidation and glutathione depletions. The animals were divided into four treatment groups with six mice in each group (n=6). A single dose of cisplatin (7 mg/kg b.w.) has been administered on the 5th day of study to cisplatin control group.  In NDGA Pre-treatment group receives cisplatin after five days NDGA pre-treatment. Then animal had been sacrificed 24 hrs after cisplatin treatment. There was a significant reduction in chromosomal aberration, glutathione level and increase in the lipid peroxidation. The anti-oxidant action of NDGA presumably modulates the oxidative stress induced by cisplatin and significantly (p<0.05) restore the reduced  level of chromosomal aberration and anti-oxidant enzymes.