Determination of antioxidant activity of the selected extracts of Mathuka Neriifolia (Moon) H.J. Lam, isolation and characterization of phytoconstituents of the ethanol extract by column chromatography

Abstract

The present study involves the determination of antioxidant  activity of the selected extracts of Mathuka Neriifolia (Moon) H.J. Lam, isolation and characterization of phytoconstituents of the ethanol extract by column chromatography.The antioxidant  potential of plant stem  extracts were investigated by hydroxyl radical scavenging activity, super oxide free radical scavenging activity, DPPH assay, nitric oxide radical scavenging activity, total antioxidant activity. Among the three extracts ethanol extract was having maximum antioxidentactivity. So the ethanol extract was subjected to column chromatography,by means of gradient elution technique.The fractions 260 – 300 and 301 – 344 ,gave two compounds A 1 and A 2.Later by spectral analysis they were found to be    Quercetin and  Luteolin 7 – glucoside.