Antibacterial activity of methanol extract of Macaranga denticulata leaves and in silico PASS prediction for its six secondary metabolites

Abstract

Antibacterial properties of methanolic extract of Macaranga denticulata leaves was studied on three Gram positive and Four Gram negative bacteria by disc diffusion method. The extract showed zone of inhibition in highest concentration of 1000 µg/ml against Gram-positive bacteria Staphylococcus aureus (Nil), Bacillus subtilis (12mm), Bacillus cereus(Nil)and Gram-negative bacteria Salmonella typhi (15mm), Salmonella paratyphi (Nil), Escherichia coli (14mm), Pseudomonas aeruginosa (14mm). Six secondary metabolites of Macaranga denticulata namely 3-acetylaleuritolic acid, oleanolic acid, macarangin, scopoletin, β-sitosterol, stigmasterol were analyzed by the PASS for their different types of biological activities and found activities like hepatoprotectant, antiulcerative, antifungal, diuretic, insulin promoter, antinociceptive, anti-inflammatory, antihypercholesterolemic, anesthetic general, bone diseases treatment and vitamin.